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    Vector Laboratories sig 31126 mom staining kit vector laboratories
    Sig 31126 Mom Staining Kit Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2774 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sig+31126+mom+staining+kit+vector+laboratories/Mouse+on+Mouse+(M%2EO%2EM%2E+)+Basic+Kit/pm40816286-281-27-31
    Average 96 stars, based on 2774 article reviews
    sig 31126 mom staining kit vector laboratories - by Bioz Stars, 2026-09
    96/100 stars

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    SYBR Green Assay:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Bicinchoninic Acid Protein Assay:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Blocking Assay:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Staining:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Mass Spectrometry:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Spatial Transcriptomics:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Western Blot:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Stable Transfection:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Expressing:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Inverse Gas Chromatography:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Quantitative RT-PCR:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Recombinant:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Bioprocessing:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5

    Luciferase:

    Article Title: ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins DMEM Gibco Cat# 10313021 L-Glutamine Gibco Cat# 25030081 Fetal Bovine Serum Gibco Cat# 10270106 Penicillin/Streptomycin Gibco Cat# 15140122 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 31985062 Blasticidin Thermo Fisher Scientific Cat# BP264725 Polybrene Sigma-Aldrich Cat# TR-1003 0.45 μm filter Thermo Fisher Scientific Cat# 15216869 cOmplete protease inhibitor cocktail Roche Cat# 11697498001 Dithiothreitol Sigma-Aldrich Cat# 43815 Novex 4-20% polyacrylamide TrisGlycine gels Invitrogen Cat# XP04205BOX NuPAGE 3-8 % Tris-Acetate gels Invitrogen Cat# EA0375BOX RNAlater Sigma-Aldrich Cat# R0901 TRIzol Invitrogen Cat# 15596026 PEI-Max Polysciences Cat# 24765-1 OCT mounting medium VWR Cat# 00411243 DAPI Sigma-Aldrich Cat# D9542 DAKO fluorescent mounting medium Agilent Cat# S3023 Proteinase K Sigma-Aldrich Cat# P2308 Protein block Abcam Cat# ab64226 VECTASTAIN Elite ABC reagent Vector laboratories Cat# PK-7100 DAB solution Abcam Cat# ab64238 DPX mounting medium CellPath Cat# SEA-1304-00A Caerulein Bachem Cat# 4030451 GSK2606414 APExBIO Cat# A3448 Hydroxypropyl methylcellulose Thermo Scientific Chemicals Cat# 04477918 HBSS Lonza Cat# BE10-527F Collagenase IA Sigma-Aldrich Cat# 2674 Trypsin inhibitor Sigma-Aldrich Cat# T6522 Waymouth’s medium Gibco Cat# 31220023 EGF Invitrogen Cat# PHG0311L MS grade water Supelco Cat# 1.15333 Lysyl endopeptidase Waiko Cat# 121-05063 MS-grade trypsin Thermo Fisher Scientific Cat# 90058 V5-Trap Magnetic Agarose beads Proteintech Cat# v5ta Myc-Trap Magnetic Agarose beads Proteintech Cat# ytma AffiniPure Fab Fragment Jackson ImmunoResearch Cat# 115-007-003 LR white resin TAAB laboratories Cat# L012 IHC Antigen Retrieval Solution Invitrogen Cat# 00-4956-58 Proteinase K solution Invitrogen Cat# AM2546 Neutral Buffered Formalin Cell Path Cat# BAF-0100-01A SSC Sigma-Aldrich Cat# S6639 Serum-free protein block Dako Cat# X0909 SYTO 13 Invitrogen Cat# S7575 Critical commercial assays RNeasy kit Qiagen Cat# 74106 qScript Cdna SuperMix Quantabio Cat# 95048 (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER SYBR green select master mix Applied Biosystems Cat# 4472920 BCA protein assay Thermo Fisher Scientific Cat# 23225 Biotin/Avidin Blocking kit Biolegend Cat# SIG-31126 MOM staining kit Vector laboratories Cat# BMK-2202 Whole Transcriptome Atlas Probe Mix Nanostring Cat# 999064 Deposited data Mass spectrometry raw data This paper PRIDE: PXD055161 Bulk mRNA-Seq raw data This paper GEO: GSE274978 Spatial transcriptomics GeoMX raw data This paper GEO: GSE274979 scRNA-Seq count matrix Ma et al. 58 GEO: GSE172380 Raw immunoblot data from figures This paper Mendeley Data: https://doi.org/10.17632/ rttwh5pj7c.1 Experimental models: Cell lines Human: Human Embryonic Kidney-293FT (HEK293FT) Clontech N/A Human: HEK293FT stably expressing REG3A-GFP This study N/A Mouse: PDAC cells (KPC model in C57/ BL6J background) Li et al. 73 N/A Mouse: PDAC cells stably expressing V5 This study N/A Mouse: PDAC cells stably expressing CCPG1-V5 This study N/A AAVpro 293T Cell Line Takara Bio Cat# 632273 Experimental models: Organisms/strains Mouse: Ccpg1 GT Smith et al. 36 MGI:5000356 Mouse: Kras LSL-G12D Jackson et al. 74 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:2429948 Mouse: Pdx1-Cre Hingorani et al. 22 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3032531 Mouse: Ccpg1 flox This paper N/A Mouse: Tp53 LSL-R172H Olive et al. 75 ; Gift from Val Brunton, IGC, Edinburgh, UK MGI:3039263 Mouse: Rosa26-LSL-cas9-P2A-EGFP Platt et al. 76 ; Gift from Kevin Myant, IGC, Edinburgh, UK MGI:5583839 Mouse: Atg5 flox Hara et al. 77 ; Gift from Komatsu Masaaki, Juntendo University, Japan MGI:3663625 Mouse: CAG-FLPe (FLPe deleter) Taconic; Gift from Ian Jackson, IGC, Edinburgh, UK MGI:3850329 Oligonucleotides For RT-qPCR primers see Table S5 N/A Recombinant DNA AAV ss-mTOLLES-Ypet (SRAI)-KDEL This paper, Subcloned from pLX304-ss- mTOLLES-YPet-KDELJimenez-Moreno et al. 48 to replace Cre in AAV-Cre 17 N/A scAAV DEST V5 This paper, Gateway destination vector for self-complementary AAV production (adapted from scAAV-GFP, Addgene #32396, a gift of John T Gray) N/A pDONR223 Luciferase-V5; Firefly Luciferase cDNA fused to V5 This paper N/A pDONR223 Reg3a; Mouse Reg3a cDNA This paper N/A pDONR223 Reg3b; Mouse Reg3b cDNA This paper N/A (Continued on next page) e4 Developmental Cell 60, 1–18.e1–e13, December 15, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER pDONR223 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pDONR223 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pDONR223 Reg3d; Mouse Reg3d cDNA This paper N/A pDONR223 Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 STOP This paper N/A scAAV Reg3b; Mouse Reg3b cDNA This paper N/A scAAV Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A scAAV Luciferase-V5; Firefly luciferase cDNA This paper N/A pDONR223 STOP This paper N/A pcDNA3.2 V5; Empty vector control This paper N/A pcDNA3.2 Reg3b; Mouse Reg3b cDNA This paper N/A pcDNA3.2 Reg3b ΔN; Mouse Reg3b cDNA (ΔG27-R37) This paper N/A pcDNA3.2 Reg3b KDEL; Mouse Reg3b fused to C-terminal KDEL motif This paper N/A pLX304 DEST V5 A gift from David Root Addgene Plasmid #25890 pLX304 DEST Reg3a-GFP; Mouse Reg3a cDNA This paper N/A pEGFP DEST C1 Smith et al. 36 N/A pEGFP; GFP only control This paper N/A pEGFP Reg3a; Mouse Reg3a cDNA This paper N/A pEGFP Reg3b; Mouse Reg3b cDNA This paper N/A pEGFP Reg3d; Mouse Reg3d cDNA This paper N/A pEGFP Reg3g; Mouse Reg3g cDNA This paper N/A pDONR223 Ccpg1 FL; Mouse Ccpg1 fulllength (1-753) Source Bioscience Cat# IRAVp968H01136D pDONR223 Ccpg1 NTD-TM; Mouse Ccpg1 (1-239) This paper N/A pDONR223 Ccpg1 ΔNTD; Mouse Ccpg1 (215-753) This paper N/A pDONR223 Ccpg1 ΔL1; Mouse Ccpg1 (1-359;582-753) This paper N/A pDONR223 Ccpg1 ΔL2; Mouse Ccpg1 (1-581) This paper N/A pDONR223 Ccpg1 ΔNTD ΔL2; Mouse Ccpg1 (215-581) This paper N/A pDONR 223 Fam134b; Mouse Fam134b Source Bioscience Cat# IMAGp998H0714447Q and IRAVp968B0235D pDONR 223 Fam134c; Mouse Fam134c Source Bioscience Cat# IRAVp968B0736D pDONR 223 Sec62; Mouse Sec62 Source Bioscience Cat# IRAVp968D06104D pDONR 223 SQSTM1 (p62); Human SQSTM1 Gift from Christian Behrends 78 N/A pDONR 223 Calcoco1; Mouse Calcoco1 Source Bioscience Cat# IRAVp968B12130D pLX304-EV; Empty lentiviral vector This paper N/A pLX304 Ccpg1-V5; Mouse Ccpg1 fulllength (1-753) This paper N/A pcDNA myc(6) DEST Created by F. Van Roy and B. Janssens, Ghent University, Belgium BCCM plasmid #LMBP 7212 pcDNA myc(6) EV; Empty vector control This paper N/A (Continued on next page) Developmental Cell 60, 1–18.e1–e13, December 15, 2025 e5



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